Modified Caspase-3 Assay Indicates Correlation of Caspase-3 Activity with Immunity of Nonhuman Primates to Yersinia pestis Infection
نویسندگان
چکیده
منابع مشابه
Caspase-12 and the Inflammatory Response to Yersinia pestis
BACKGROUND Caspase-12 functions as an antiinflammatory enzyme inhibiting caspase-1 and the NOD2/RIP2 pathways. Due to increased susceptibility to sepsis in individuals with functional caspase-12, an early-stop mutation leading to the loss of caspase-12 has replaced the ancient genotype in Eurasia and a significant proportion of individuals from African populations. In African-Americans, it has ...
متن کاملSurvivin does not inhibit caspase-3 activity.
Conway et al recently reported that alternatively spliced forms of mouse survivin exhibit different antiapoptotic properties.1 This was inferred from inhibition of recombinant caspase-3 catalytic activity using a standard chromogenic assay in the presence of increasing concentrations of mammalian-expressed murine survivin isoforms of 140, 121, and 40 amino acids, respectively.1 If confirmed, th...
متن کاملCaspase-3 activity in papillary thyroid carcinomas.
AIM The aim of this work was to assess caspase-3 activity in the tissue of papillary thyroid carcinomas of patients and analyze the peculiarities of changes in this activity depending upon a number of pathomorphological and clinical features of tumoral process. METHODS Caspase-3 activity was determined by spectrophotometry with regard to acetyl-asp-glu-val-asp-paranitroanilide. RESULTS At i...
متن کاملSendai virus infection induces apoptosis through activation of caspase-8 (FLICE) and caspase-3 (CPP32).
Sendai virus (SV) infection and replication lead to a strong cytopathic effect with subsequent death of host cells. We now show that SV infection triggers an apoptotic program in target cells. Incubation of infected cells with the peptide inhibitor z-VAD-fmk abrogated SV-induced apoptosis, indicating that proteases of the caspase family were involved. Moreover, proteolytic activation of two dis...
متن کاملSensing caspase 3 activity with quantum dot-fluorescent protein assemblies.
We demonstrate the use of a hybrid fluorescent protein semiconductor quantum dot (QD) sensor capable of specifically monitoring caspase 3 proteolytic activity. mCherry monomeric red fluorescent protein engineered to express an N-terminal caspase 3 cleavage site was ratiometrically self-assembled to the surface of QDs using metal-affinity coordination. The proximity of the fluorescent protein to...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical and Vaccine Immunology
سال: 2008
ISSN: 1556-6811,1556-679X
DOI: 10.1128/cvi.00091-08